User manual for aml oceanographics plus x 8 led indicator the led indicator is located next to the datapower connector on the instruments top endcap. For safety and biohazard guidelines, see the safety appendix in the yfiler plus pcr amplification kit user guide pub. The yfiler plus pcr amplification kit uses the same improved process for synthesis and purification of the amplification primers that are previously developed for other nextgeneration thermo fisher scientific str chemistries. Applied genetics rapidly mutating rm ystrs dys576 dyf399s1 dyf387s1 dys570 multiplex 1 multiplex 2 dys526a dys626 dys526b dys627 dys518 multiplex 3 dys612 dys449 dys547 dyf404s1 dyf403s1a dyf403s1b. The yfiler plus pcr amplification kit is designed for forensic laboratories that. Therefore, we investigated the 17 yfiler ystrs in 1,7301,764 dnaconfirmed. For each of these six panels differentsized databases exist. Table of % minus and % plus stutter which includes minimum, maximum, and mean stutter values nontechnical changes. The systemsure plus is designed for convenient, singlehand use to make using the system and navigating the menus easy for every user.
Nanodrop user manual nanodrop user manual, agilent bioanalyzer rna isolationcleanup qiagen rneasy plus mini column manual, mirna. The system includes loci with tri, tetra, penta and hexanucleotide repeats and. Pdfxchange editor is one of the fastest and most featurerich options available worldwide to view, edit, modify and ocr imagebased pdf files. Indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury. Note that smallersized panels are included in the next larger panels. Plusx endcaps are available with different combinations of ports to allow for a wide variety of sensor combinations. Forensic genetic value of a 27 ystr loci multiplex. Ampflstr yfiler pcr amplification user guide thermo fisher. Software library for tracked ultrasound image acquisition, calibration, and processing. Str results interpretation identifiler and yfiler status. Comprehensive mutation analysis of 17 ychromosomal short. Tennessee bureau of investigation forensic services. Applied biosystems quantifiler trio, yfiler plus and identifiler kits. User manual installation instructions customer service booklet warranty enclosure information on the energy consumption and noises technical specifications refrigerant, cubic capacity and further technical specifications can be found on the rating plate.
Internal validation of the ampflstr yfiler amplification. A total of 197 unique haplotypes singletons were obtained from 203 individuals using the 27 ystr yfiler plus kit. Collect all the information on each of the available loci. Any user of the site can also choose to advertise their business on. Plus applications include complete enduser software applications for spatial and temporal calibration fcal and realtime data. Figure 2 1 ng of control dna 007 amplified with the yfiler kit and analyzed on the applied biosystems 3xl genetic analyzer. These products may be covered by one or more limited use label licenses. If youre the savvy type who cant wait to explore the app right away, please feel free to do so, although youre invited to read through this guide first to get a general picture of what the app is capable of.
Y str panel european minimal haplotype plus dys438 and dys439. Yfiler plus user manual keyword after analyzing the system lists the list of keywords related and the list of websites with related content, in addition you can see which keywords most interested customers on. For the 52 samples where a yfiler plus profile was obtained, the number of alleles recovered in each sample was compared to that achieved with yfiler. Indicates a potentially hazardous situation which, if. Developmental validation of a novel 5 dye ystr system. User manual 490 kb download cardioline ar2100adv user manual 480 kb download cardioline ar2100view user manual 480 kb download cardioline ar2100viewbt user manual 780 kb download cardioline delta 1 plus ecg recorder user manual 1. Afterwards, the wholegenomeamplified dna was purified using invisorb 96 filter microplates invitek gmbh, berlin, germany. Amplification using the yfilertm system 1general information for amplification 1.
Overpressure can result in damage to the pxchange sensor and the instru ment. Plus applications user manual documentation introduction plus public software library for ultrasound is an opensource software package for tracked ultrasound image acquisition, calibration, and processing. The database can be searched either using manual input or by uploading files, including genemappergenotyper export files figure 7. Ampflstr yfiler pcr amplification kit user s manual vii preface how to use this guide purpose of this guide the ampflstr yfiler pcr amplification kit user s manual provides information about the applied biosystems instruments, chemistries, and software associated with the ampf lstr yfiler pcr amplification kit yfiler kit. Validation of the yfiler plus pcr amplification kit for. This equipment has been tested and found to comply with the limits for a class b digital device, pursuant to part 15 of the fcc rules. Users manual tobii dynavox pceye explore tobii dynavox pceye explore users manual v. The robust manual mode gives you control over every aspect of your image. Ystr haplotype reference database yhrd is a searchable worldwide database of ystr haplotypes in various formats along with tools build upon their distribution. Experiments were performed using two sets of yfiler amplification parameters. Yfiler plus pcr amplification kit using a new 6dye system detects 27 ystr loci. This paper describes our validation results of the yfiler plus kit for forensic casework analysis. These limits are designed to provide reasonable protection against harmful interference in a residential installation.
L total reaction volume of the standard protocol but the recommended taq concentration was doubled and thus a maximum volume of 9. Several validation studies have proved the yfiler plus kit yield high. Donna foskin from esr describes optimization for processing sexual assault kits using forensic dna analysis. Systemsure plus atp monitoring system the worlds best. The led indicator will be on whenever the datapower cable is plugged into the instrument. Ampflstr yfiler pcr amplification kit users manual vii preface how to use this guide purpose of this guide the ampflstr yfiler pcr amplification kit users manual provides information about the applied biosystems instruments, chemistries, and software associated with the ampf lstr yfiler pcr amplification kit yfiler kit. The list of the ystr haplotypes observed in the four italian geographic areas is reported in supplementary table 1. Dys437, dys448, dys456, dys458, dys635 y gata c4, and y gata h4. User guide yfiler plus pcr amplification kit for use with. A screen resolution of less than this will mean that solo plus will not display correctly and you will not be able to see all of the information. A total of 28 questioned samples, 20 male reference samples, 7 female reference samples, 19 reagent blanks and blind control samples were amplified with the yfilertm kit. Ystr panel european minimal haplotype plus dys438 and dys439. Plus public software library for ultrasound is an opensource software package for tracked ultrasound image acquisition, calibration, and processing. A total of 27 y chromosomal short tandem repeat str loci were amplified with the yfiler plus pcr amplification kit from 2314 unrelated healthy male individuals of zhuang ethnic minority living.
Jan 21, 2009 for standard reactions, volumes and amplification conditions as defined in the ampf. Read the safety data sheets sdss and follow the handling instructions. Evaluation of modified yfiler amplification strategy for. The system and software allow you to print great models with only a few keystrokes used a 3d printer before. User manual overview of the main unit a a preset select a preset radio station. Overview the up plus 3d printer is designed with ultimate portability and simplicity in mind. Prologue the most efficient way to use this manual is to first read section iioverview by reading section ii, you will gain a general knowledge about how vfiler fits into the. The yfiler plus pcr amplification kit is designed for forensic laboratories that process highly challenging ystr casework and database samples. Indicates a potentially hazardous situation which, if not avoided, may result in serious injury. Assessment of the yfiler plus pcr amplification kit for. Applied genetics national institute of standards and. Pdfxchange editor plus user manual pdfxchange editor is one of the fastest and most featurerich options available worldwide to view, edit, modify and ocr imagebased. Appendix q quality control check of yfiler plus kit. Periodically perform scheduled maintenance on the device to avoid dirt buildup that could compromise the correct, safe operation of the unit.
Manual tune to tune to a specific channel manually. Forensic genetic value of a 27 ystr loci multiplex yfiler. We made every effort to ensure that this users manual is accurate and complete. Qiacube follows the same steps as the manual procedure i. Learn about how to use file viewer plus with the official file viewer plus 3 user manual. Page 36 in the image on the left of a green curve, the red indicates the amount of support material, changing the angle higher will produce more support. Dont let the petite shape fool you systemsure plus makes no compromises when it comes to durability. Do not exceed the specified pressure ratings of the pxchange sensor or instrument housing. Yfiler plus pcr amplification kit user guide thermo fisher scientific. Yfiler plus pcr amplification kit thermo fisher scientific. Diversity indexes for the entire sample and for the four areas are reported in table 1 and 2, respectively. Subsequently, 9l reaction buffer plus 1 l of enzyme mix were added to the cooled sample and incubated at 30c for 1618 h, then heat inactivated at 65c for 10 min.
Powerplex y12 12, yfiler 17, powerplex y23 23, yfiler plus 27 and maximal 29 figure 8. However, no liability is assumed for any errors and omissions that may have occurred. The yfiler plus kit is now approved for use by laboratories generating dna profiles for inclusion in the us national dna index system ndis codis database. User manual for aml oceanographics plus x 6 plusx endcap with one primary and four secondary ports abbreviated as p1s4. Ychromosomal shorttandem repeat ystr amplification has been used in forensic casework at the bureau of criminal apprehension bca forensic science laboratory since 2003.
Str yfiler pcr amplification kit users manual were followed. If youre the savvy type who cant wait to explore the app right away, please feel free to do so, although youre invited to read through this guide first to get a general picture of what the app is. Product description the yfiler kit contains all the necessary reagents for the amplification of human malespecific genomic dna. Internal validation of the applied biosystems ampflstr yfiler. Of these 52 samples, 28 54% yielded a yfiler plus profile defined as the presence of at least one allele when a yfiler profile had not previously been obtained. This operating manual is divided into many sections and presented in a logical order assuming that it will be read section by section following the examples. Make sure that there is an easilyaccessible outlet with a capacity of no less than 10a closely to where the device is to be installed. Internal validation of the ampflstr yfiler amplification kit. Yfiler plus pcr amplification kit pcr setup swab substrate catalog numbers 4484678 and 4482730 pub. Str yfiler pcr amplification kit users manual were followed 25. The oneplus 3t is equipped with dual stabilization technologies. Easy link plus series dvr about this manual the material in this document is for information purpose and is subject to change without prior notice. The pdfxchange editor v6 user manual is composed of the following sections.
Semisolid honeycomb up plus 2 3d printer user manual v 20. For standard reactions, volumes and amplification conditions as defined in the ampf. Developmental validation following swgdam guidelines of the yfiler plus pcr amplification kit 6dye, 27plex ystr kit that includes the 17 loci from the yfiler kit plus 10 new loci including 7 rapidly mutating ystrs for improved discrimination of related individuals. Wear appropriate protective eyewear, clothing, and gloves. Your product is designed and manufactured with high. Yfiler plus pcr amplification kit pdf free download.
Yfiler plus amplification kit validation and calculation of. This user guide describes all the functions available in the notes plus app. The yfiler plus pcr amplification kit has been validated with data that was analyzed using both. To date, 17 ystr loci included in the yfiler pcr amplification kit. Open evoware standard software and follow prompted instructions. Qiagen gmbh, qiagen strasse 1, 40724 hilden, germany qiagen kit handbooks and user. Procedure manuals virginia department of forensic science. Ampflstr yfiler pcr amplification kit users manual pn 4358101c. The front camera will dynamically adapt to lowlight.